In addition to tobacco and alcohol abuse, certain viruses have been associated with squamous cell carcinoma SCC of the head and neck, causing alterations in DNA. It has been demonstrated that the human papillomavirus HPV type 16, a subtype of the human papillomavirus, is present in the oropharyngeal carcinomas of non-smokers patients inclusive. HPV-infected cells express some viral proteins encoded by genes called E6 and E7, and can inactivate p53 protein and the retinoblastoma-type protein RBP involved in human papillomavirus on the tongue regulation of proliferation and cell death.
Materials and method. We present an immunohistochemical study conducted to identify significant tumour markers in tonsillar SCC. We present the statistically significant correlations between the presence of immunohistochemical markers and studied local recurrence, lymph node recurrence and risk of a second cancer in the aerodigestive upper tract.
The demonstration of HPV in tonsillar tumour tissue requires in situ hybridization or polymerase chain reaction PCR for the evidence of viral genome included into the host cell. The practical implications of an etiologic role of HPV in head and neck cancer generally and in tonsillar SCC in particular remains in question and is in relate with prognosis, treatment and prevention.
În afară de consumul de tutun şi abuzul de alcool, anumite virusuri au fost asociate cu carcinomul cu celule scuamoase CCS al capului şi gâtului, cauzând alterări la nivelul ADN-ului.
Este dovedit că virusul papiloma uman HPVtipul 16, este prezent la nivelul carcinoamelor orofaringiene inclusiv în cazul nefumătorilor. Celulele infectate cu HPV exprimă unele proteine virale codate de genele denumite E6 şi E7 şi pot inactiva proteina p53 şi proteina de tip retinoblastom RBP implicate în reglarea proliferării şi morţii celulare.
Materiale şi metodă. Prezentăm un studiu imunohistochimic realizat cu scopul de a identifica markeri tumorali semnificativi în CCS de amigdală.
Head and Neck Cancer: Eric M. Genden · | Books Express
Prezentăm corelaţiile semnificative statistic între prezenţa markerilor imunohistochimici şi recurenţa locală, recurenţa nodulilor limfatici şi riscul apariţiei unui al doilea cancer în tractul aerodigestiv superior. Punerea în evidenţă a HPV-ului în ţesutul tumoral amigdalian necesită hibridizare in situ şi reacţie de polimerizare în lanţ PCR pentru punerea în evidenţă a genomului viral conţinut în celula-gazdă. Implicaţiile practice ale unui rol etiologic al HPV-ului în cancerele de cap human papillomavirus on the tongue gât, în general, şi în CCS de amigdală, în human papillomavirus on the tongue, reprezintă un subiect în dezbatere, fiind în relaţie cu prognosticul, tratamentul şi prevenţia acestor tipuri de cancere.
Cuvinte cheie carcinomul cu celule scuamoase de amigdală CCS HPV markeri tumorali Introduction The tonsillar squamous cell carcinoma SCC is becoming a public health problem because of its rising incidence in the last 20 years, in contrast to the decreasing incidence of carcinomas in other subsites of head and neck associated to the reduced prevalence of smoking.
These tumours of oral cavity, oropharynx, larynx, hypopharynx and sinonasal region are linked by common characteristics, including a male predominant appearance in the 5th-6th decade of life, an important etiological link with tobacco, alcohol use or betel nut chewing, and a histopathological resemblance 1.
HUMAN PAPILLOMA VIRUS GENOTYPING IN FRESH HEAD AND NECK TUMORS - OUR FIRST EXPERIENCE
Data regarding the epidemiology revealed that in Romania the oropharyngeal cancer represents 2. In France, during the last 30 years, the mortality in oral and oropharyngeal cancer increased by three times 1. As in cervical cancers, the oropharyngeal infection with HPV is a human papillomavirus on the tongue transmitted disease which involves some particularities of sexual behaviour: a large number of vaginal sex partners, oral and anal sex.
The recent increasing of OPSCC incidence may reflect the social changes regarding sexual behaviour in the modern world 6. The anatomical sites preferred by HPV in oropharynx are the tonsils and the tongue, because of the unique presence of transitional mucosa in human papillomavirus on the tongue and particular in tonsillar tissue, which curatarea ficatului cu sare amara important histological similarities with the cervical mucosa.
Tonsillar epithelium invagination may favour virus capture and promote its access to basal cells the only dividing cells in the epithelium. The tonsillar tissue could be a reservoir for HPV in the upper aero digestive tract.
We had human papillomavirus on the tongue premises for our study on tonsillar cancers. The second consists in the fact that mutagens such as tobacco, alcohol and HPV viral oncogenes E6 and E7 induce dysfunctions of two major mechanisms of cellular cycle, which involves the p53 and RBP tumoral suppressor genes 2.
Materials and method We made an immunohistochemical retrospective study between andaiming to identify any correlations between tumoral markers and human papillomavirus on the tongue evolution and prognosis in tonsillar SCC. Materials We studied 52 cases of patients diagnosed with tonsillar SCC.
We had a first group Group I with 25 cases, where the positive diagnose was made by biopsy and these patients had radiotherapy as first curative method of treatment. We had a second group Group II with 27 cases, where the positive diagnose was made on surgical specimens and these patients had surgery as the first curative method of treatment. The two groups were similar regarding age and gender distribution.
The dilutions and markers specifications are revealed in Table 1. We also studied lymphocyte populations CD4, CD8, and populations of dendritic cells in tumour tissue. Table 1. The dilutions and markers specifications For the immunohistochemical identification of tumoral antigens we used the three-stadial indirect method Avidine-Biotine-Peroxidase ABPafter Hsu and colab.
Results The gender repartition of cases was: 47 male cases and 5 female cases. The age repartition of cases was: two cases between years old, 14 cases between years old, 21 cases between years old, 10 cases between years old, and five cases between years old. The correlation coefficient between the two sets of data, corresponding to Group I and Group II, was 0. In both groups, we had 48 smoker patients, human papillomavirus on the tongue The patients who were both smokers and alcohol consumers represented